Closed FC 800-C

Closed FluorCam FC 800-C represents a highly innovative, robust and user friendly world-wide used system for combined multispectral and kinetic fluorescence imaging.

  • Product Name:Closed FC 800-C
  • Item No.:FC 800-C
  • Manufacturers:PSI
  • Country of Origin:Czech Republic

Closed FluorCam FC 800-C represents a highly innovative, robust and user friendly world-wide used system for combined multispectral and kinetic fluorescence imaging. It consists of a CCD camera, four to five fixed LED panels (4 + 1 additional, which is not included in the standard setup) and, optionally, of a filter wheel equipped with up to 7 different emission filters. The LED panels in size of 13 x 13 cm provide uniform irradiance over sample area – suitable for imaging of small plants (such as Arabidopsis thaliana), detached leaves, mosses, lichens, plated algal colonies or multi-well plates with algal suspensions, etc. The system is very compact and allows easy dark adaptation of an investigated sample.

The Closed FluorCam FC 800-C generates images of fluorescence signal at any moment of the experiment and presents them using a false color scale. Full kinetic analysis is available. In all applications, the camera allows imaging of fluorescence transients that are induced by actinic light or by saturating flashes. The timing and amplitude of actinic irradiance are determined by user-defined protocols.

Standard version of the FluorCam system includes four super bright LED panels 13 x 13 cm. One pair of LED panels provides Measuring Light and Actinic Light 1 (red-orange 617 nm in standard setup). Other two panels provide Actinic Light 2 and Saturating Pulse (in standard version cool white, typically 6500 K). Other available (but optional) wavelengths for light sources are: royal-blue (447 nm), blue (470 nm), green (530 nm), cyan (505 nm), red (627 nm), deep-red (655 nm), amber (590 nm) - these lights sources are only for customized FluorCam versions. Additional LED panel may be added to increase the colour spectrum of the light sources for multi-colour fluorescence measurements. Additional panel is mounted around the camera. Available colours are: far-red (740 nm), deep-red (660 nm), ultra-violet (365 or 385 nm), green (523 nm), amber (590 nm). Other options available upon request.

The FluorCam FC 800-C also includes a high-performance PC and comprehensive software package including full system control, data acquisition and image processing. For an experienced professional, the software offers a sophisticated programming language that can be used for designing novel timing and measuring sequences.


Fluorescence Parameters:

- Measured parameters: FO, FM, FV, FO', FM', FV', FT

- More than 50 calculated parameters: FV/FM, FV'/FM', PhiPSII , NPQ, qN, qP, Rfd, PAR-absorptivity coefficient, and many others

Light Sources:

Standard: red-orange (617 nm), cool white (typical colour temperature 6500 K)

Optional: royal-blue (447 nm), blue (470 nm), green (530 nm), cyan (505 nm), red (627 nm), deep-red (655 nm), amber (590 nm)

Super Pulse Intensity:

- 4,000 µmol(photons)/m².s (in standard version)

- 6,000 µmol(photons)/m².s (in light-upgraded version)

Actinic Light Intensity:

Up to 2,000 µmol(photons)/m².s (in standard version)

Up to 3,000 µmol(photons)/m².s (in light-upgraded version)

Filter Wheel:

7 positions

Light Regime:

Static or dynamic (sinus form)

Custom-Defined Protocols:

Variable timing, special language and scripts

CCD Detector Wavelength Range:

400 – 1000 nm

CCD Format:

- 720 x 560 pixels (TOMI-1 camera)

- 1360 x 1024 pixels (TOMI-2 camera)

- 1280 x 1024 pixels (TOMI-3 camera)

Pixel Size:

- 8.20 µm x 8.40 µm (TOMI-1)

- 6.45 µm x 6.45 µm (TOMI-2)

- 6.60 µm x 6.60 µm (TOMI-3)

A/D bit Resolution:

- 12 bit (TOMI-1)

- 16 bit (TOMI-2)

- 12 bit (TOMI-3)

Spectral Response:

QE max at 540 nm (~70 %), 50 % roll-off at 400 nm and 650 nm

Read-Out Noise:

Less than 12 electrons RMS - typically only 10 electrons

Full-Well Capacity:

Greater than 70,000 electrons (unbinned)

Imaging Frequency:

Maximum 50 frames per second

Bios:

Upgradeable firmware

Interface Connector:

Gigabit Ethernet

Outer Dimension:

471 mm (W) x 473 mm (D) x 512 mm (H)

Inner Dimension:

450 mm (W) x 450 mm (D) x 400 mm (H)

Weight:

Appr. 36 kg

Power Input:

Appr. 1100 W

Electrical:

90 – 240 V