FluoroProbe Yellow Substances and automatic Turbidity Correction

The chlorophyll measuring instrument with algae class differentiationThe bbe FluoroProbe is a highly sensitive measuring instrument for the analysis of chlorophyll with algae class determination. Indi

  • Product Name:FluoroProbe Yellow Substances and automatic Turbidity Correction
  • Maunufactor:BBE

The chlorophyll measuring instrument with algae class differentiation

The bbe FluoroProbe is a highly sensitive measuring instrument for the analysis of chlorophyll with algae class determination. Individual profiles during the measurements are taken for green algae, blue-green algae/cyanobacteria, diatoms/dinoflagellates and cryptophytes.

This enables the analysis of the occurrence and distribution of e.g. blue-green algae on site without the necessity of the laboratory. Possible interferences due to yellow substances are eliminated by an integrated CDOM correction factor.

Chlorophyll in vivo measurement can be affected by particulate matter in the water body.  Therefore a correction is essential when the turbidity is too high. The bbe FluoroProbe  with the optional transmission sensor provides automatic turbidity correction using the latest bbe++ software extension. Turbidity correction with the FluoroProbe makes chlorophyll determination even more reliable.

The bbe FluoroProbe can quickly and reliably determine the chlorophyll content of different algae classes down to a depth of 100m (optionally 1000 m). The measurement data can be displayed in real time on a PC or be stored in the probe for subsequent evaluation.

Measurement principle

The fluorescence of algae (emission at approx. 700nm wavelength) due to excitation by visible light mainly depends on the presence of chlorophyll-a, a common pigment in the plant world. The occurrence of other pigments is typical for the different algae classes. Interactions between these different pigment systems with chlorophyll-a result in a special excitation spectrum for the taxonomical algae classes.

The special patterns of this algae fluorescence - so-called fingerprints - are used in the bbe fluorometers for the quantification of different algae classes. The excitation light sources are LEDs with different wavelengths. The fingerprints of four algae classes and of yellow substances are already stored in the FluoroProbe. Special user-defined fingerprints can also be added

Advantages of the FluoroProbe

  • determination of green algae, blue-green algae (cyanobacteria), diatoms and cryptophytes

  • up to 4 other algae classes can be calibrated by the user

  • up to 4 measurements per second

  • yellow substances correction and compensation of interferences by UV-LED

  • turbidity correction

  • reduces the number of required microscopic laboratory examinations

  • internal rechargeable batteries

  • internal datalogger

  • PC software for data analysis

  • options: pressure sensor, transmission measurement

  • total chlorophyll [µg chl-a/l]

  • concentration of green algae [µg chl-a/l]

  • concentration of cyanobacteria [µg chl-a/l]

  • concentration of diatoms/brown algae [µg chl-a/l]

  • concentration of cryptophytes [µg chl-a/l]

  • fingerprints of classes/species, also user-defined [µg chl-a/l]

  • yellow substances

  • depth

  • temperature (optional)

  • transmission (optional)

Chlorophyll0 - 500 µg chl-a/l
Resolution0.01 µg chl-a/l
Transmission0 - 100%
TemperatureSample: -2 - 40°C / Environment: -2 - 40°C
Housing materialreinforced carbon fibre/V4A steel
Weight6.4 kg (7.2 kg with lightshield)
Dimensions (H x D)450 x 140 mm
Voltage12 V
Battery capacity3900 mA h
Operating timecontinuously up to 10 h; interval up to 30 days
Memory capacity2 GB memory card, 10 million datasets
Maximal diving depth
  • 0 - 100 m (standard)

  • 0 - 200 m (extended range)

  • hard plastic case

  • power supply

  • protection cap

  • USB adapter

  • USB stick

  • manual

  • software

  • Temperature measurement

  • Transmission measurement (turbidity)

  • Cable lengths of 2, 5, 10, 20, 30, 50 and 100m

  • Workstation 25 (benchtop holder)

  • Wydro-Wiper Unit

  • Flow-Through Unit

  • Protective Cage

  • SDI-12 with bbe converter