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Fluorescence Kinetic Microscope

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Fluorescence Kinetic Microscope (FKM) is designed to be the most versatile tool for lab-based research. It extends the complete capacity of kinetic chlorophyll or multicolor fluorescence imaging to the realm of individual cells and sub-cellular structures. All conventional fluorescence parameters can be mapped with micro-meter resolution so that individual chloroplasts or even grana-stroma thylakoid segments can be investigated. The modular setup of the FKM is designed for kinetic fluorescence measurements with various user-selectable excitation and emission wavelengths as well as the combination of imaging measurements with spectrally resolved or ultra fast (μs) spot measurements of fluorescence and absorbance kinetics.

The FKM allows imaging measurements of two-dimensional resolved multicolor fluorescence transients induced by complex irradiance protocols. The fluorescence emission is induced by an appropriate LED light source.

In the standard configuration, the wavelength selection is the same as in other research-grade fluorescence microscopes by the use of a white excitation light source combined with a set of excitation filters, dichroic mirrors, and emitter filters. As an additional option, a light source with tuneable spectra for both measuring and actinic/saturating light is available. It increases the range of measurable chromophores and improves signal/noise ratio. The system is designed to allow automatic switching between different excitation wavelengths even during same measurement. In contrast to conventional fluorescence microscopes, the ultrahigh sensitivity of the measuring camera combined with modulated light measurement makes it possible to perform imaging at extremely low light levels that do not disturb the metabolism of the cell.

The Fluorescence Kinetic Microscope can be combined with spectrally resolved measurements which are done via the Spectrometer SM 9000 synchronized to the measuring camera .The ultrafast kinetic measurements are performed by a microscope-adapted version of the Double Modulation Fluorometer FL 3500, which again can be synchronised to the measuring camera. In this way, the spectrally resolved or ultrafast kinetic spot measurements can be done simultaneously on the same object and controlled by the same measuring protocol as the imaging kinetic record.

All parts and functions can be controlled by the FluorCam software depending on individual configuration

 

Device Description: 1: Measuring camera; 2: 3-port motorised video adapter; 3: 2-port manual switching adapter; 4: Fibreoptics adapter for spectrometer; 5: Spectrometer; 6: C-mount adapter for photo camera; 7: Motorised wheel for filter cubes (8 cubes); 8: filter wheel for switching excitation while maintaining emission filters; 9: FKM control unit; 10: Peristaltic pump for liquid media; 11: Air pump; 12: Temperature control unit; 13: Flow-through thermostat.

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