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OX1LP-1mL Dissolved O2 Package

Qubit

The OX1LP-1mL Dissolved Oxygen Package may be used to measure photosynthesis and/or respiration in the aqueous phase, using suspensions of plants, animals and organelles (chloroplasts and mitochondria). It may also be used to monitor chemical and biochemical reactions that either produce or consume O2 in the aqueous phase. The Dissolved Oyxgen Package comes complete with instruments, software and interface.

The OX1LP-1mL Dissolved Oxygen Package includes:

  • G108 Dissolved Oxygen Cuvette Electrode (1 mL)
  • A231 Cuvette Electrode Amplifier
  • A255 Magnetic Stirrer
  • A135 Stir Bar (2) and O-Ring (2)
  • A131 KCl Filling Solution
  • A136 Membrane Material and Filter Paper
  • 10 ft Excelon Tubing for the Water Jacket
  • C410 LabPro Interface
  • C901 Logger Pro Software
  • C404 Customized Setup Software
  • Instructor’s and Student’s Manuals

The OX1LP-1mL Dissolved Oxygen Package has features included in no other polarographic O2 monitoring system. The O2 electrode within the water-jacketed cuvette may be used for measurements of O2 consumption (or production) by biological samples or enzymatic reactions. Calibration is a simple 2-point process, and all the requirements for calibration are supplied as part of the package.

For many O2 electrodes, changing the membrane is a tedious and awkward process. Not so with Qubit Systems’ O2 electrode. The membrane is located under the cuvette, which screws onto the base plate, and requires only a few seconds to change. All materials for replacing the membrane (filter paper and membrane material) are included in the Dissolved Oxygen Package.

Using C901 Logger Pro software, the O2 electrode may be calibrated in units of mg O2/L, % dissolved O2, ppm, or whatever units are appropriate for your study. The Dissolved Oxygen Package provides excellent accuracy and a resolution of 0.1% O2 (where air saturated water = 100%). Dissolved O2 values may be expressed in any desired units (mg/L; % dissolved O2; ppm etc) based on a simple calibration procedure. The water jacket allows calibrations and experiments to be carried out at the same temperature, or a simple temperature correction can be made in software for measurements made at different temperatures.

Applications:

The Dissolved Oxygen Package may be used for any application that involves measurement of O2 in aqueous solutions. It can be used in the laboratory for measurement of O2 concentrations from samples taken from rivers and lakes, or to measure O2 consumption (or production) by living organisms. The Dissolved O2 Package is a perfect complement to Qubit Systems’ RespirationPlant CO2 Analysis and Photosynthesis Packages. While these packages are designed to measure the exchange of respiratory and photosynthetic gases in the gas phase, the O2 electrode allows similar measurements to be made on aquatic organisms, and also on isolated organelles, such as mitochondria and chloroplasts.

Experiments Include:

  • Measurement of Aquatic Photosynthetic O2 Evolution
  • Effect of Inhibitors on Mitochondrial Electron Transport
  • Analysis of Light-dependent Chloroplast Electron Transport
  • Effects of Temperature on O2 Uptake by Aquatic Animals
  • Kinetics of O2-consuming Enzymes

Optional Components:

  • Syringes for addition of metabolites to cuvette; (A141) 10µL, (A143) 50µL, (A145) 100µL
  • A111 Variable halogen light source
  • S141 Light sensor
  • S172 Thermistor is not available with the OX1LP-1mL package due to size constraints

 

Experimental data showing the oxygen consumption rate of Glucose Oxidase using the OX1LP Dissolved Oxygen Package:

Other OX1LP Packages:

References:

  • Sokolova IM, Sokolov EP, Ponnappa KM. Cadmium exposure affects mitochondrial bioenergetics and gene expression of key mitochondrial proteins in the eastern oyster Crassostrea virginica Gmelin (Bivalvia:Ostreidae).  Aquatic Toxicology 73:241-255 (2005)
  • Lanniga G, Cherkasova AS, Sokolova IM. Temperature-dependent effects of cadmium on mitochondrial and whole-organism bioenergetics of oysters. Marine Environmental Research (Apr 2006)
  • Iris Bauer and Andreas Kappler. Rates and Extent of Reduction of Fe(III) Compounds and O2 by Humic Substances. Environ. Sci. Technol. Vol 43, Number 13, p4902–4908 ( 2009).
  • Carrie M. Mosher, Matthew A. Hummel, Timothy S. Tracy and Allan E.  Rettie. Functional Analysis of Phenylalanine Residues in the Active Site of Cytochrome P450 2C9. Biochemistry Vol 47, Number 45, p11725–11734 (2008).
  • Bryce A. Mendelsohn, Bethany L. Kassebaum and Jonathan D. Gitlin. The zebrafish embryo as a dynamic model of anoxia tolerance.  Developmental Dynamics Vol 237, Issue 7, p1780–1788 (2008).
  • Bryce A. Mendelsohn and Jonathan D. Gitlin. Coordination of development and metabolism in the pre-midblastula transition zebrafish embryo.  Developmental Dynamics Vol 237, Issue 7, p1789–1798 (2008).
  • Johnson EA, Rosenberg J, McCarty RE.  Expression by Chlamydomonas reinhardtii of a chloroplast ATP synthease with polyhistidine-tagged beta subunits.  Biochimica et Biophysica Acta 1767:374-380 (2007)
  • Johnson E A. Altered expression of the chloroplasts ATP synthase through site-directed mutagenesis in Chlamydamonas Reinhardtii.  Photosynth Res vol 96:153-162 (2008)
  • Charles W. Locuson, Peter M. Gannett and Timothy S. Tracy. Heteroactivator effects on the coupling and spin state equilibrium of CYP2C9. Archives of Biochemistry and Biophysics Vol 449, Issues 1-2, p115-129 (2006).

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