Fast Fluorometer FL 3500-F

The Fast Fluorometer FL 3500-F has multiple novel features, first of all the time resolution of 1 µs, that enable combining PAM measurements with fast kinetic measurements of OJIP and of flash fluorescence induction.

  • Product Name: Fast Fluorometer FL 3500-F
  • Item No.: Fast Fluorometer FL 3500-F
  • Manufacturers:PSI
  • Country of Origin:Czech Republic

The Fast Fluorometer FL 3500-F has multiple novel features, first of all the time resolution of 1 µs, that enable combining PAM measurements with fast kinetic measurements of OJIP and of flash fluorescence induction. Minimum detection limit is 1 µg Chla/l. Maximum detection limit depends on used protocol, it is approximatelly 26 mg Chla/l. 

The core of the instrument is the measuring optical head containing a standard cuvette for sample suspensions (10 x 10 mm base, up to 4 ml of internal volume). The measuring head is supplied with three sets of light-emitting diodes and a PIN diode detector with 500 kHz/16-bit AD converter. Gain and integration time of the converter are software controlled. The detector can measure Chl-fluorescence signal with time resolution up to 1 µs. The instrument has two input channels.

The Fluorometer is supplied with three, optionally four, sets of light-emitting diodes that generate: 

Measuring flashes, typically 2-5 µs long. Standard color: red-orange, λmax=617 nm and blue, λmax=455 nm.

Single-turnover saturating flashes, typically 20-50 µs long. Standard color: red, λmax=630 nm (optionally blue λmax=455 nm; just one color possible for single-turnover saturating flashes).

Continuous actinic light. The maximum intensity is 3,000 µmol(photons).m-2.s-1. Standard color: red, λmax=630 nm (optionally blue λmax=455 nm; just one color possible for continuous actinic light).

Additional far-red light (λmax=735 nm) for Photosystem I excitation (optional).

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Fluorometer FL 3500 Light Scheme / Spectral Characteristics

Light intensities and timing are software controlled separately for each LED set. The data are processed and the instrument is controlled by the FluorWin software (Windows 95/98/2000/XP compatible). As an option, the customer can also choose light-emitting diodes of different wavelengths. Contact PSI for your specific needs.


KEY FEATURES:

  • Detection limit 1 µg Chla/l

  • Four sets of light-emitting diodes – measuring light blue, measuring light red, single-turnover saturating light red, actinic light red

  • Light intensities and timings are software controlled separately for each LED set with 100 ns resolution

  • PIN photodiode detector with 40x variable gain for signal acquisition with 1MHz/16-bit maximum accuracy

  • The instrument capacity can be enhanced by accurate temperature regulation provided by the PSI Thermoregulator and Magnetic Stirrer


Measured Fluorescence Parameters:

F0, FM, FV, F’0, F’M, F’V, FT

Light Sources:

620 nm and 460 nm in standard versions; other wavelengths available as an option

Super Pulse Irradiance:

Around 80,000 µmol(photon).m-2.s-1 - adjustable from 0 to maximum

Actinic Light Irradiance:

Up to 3,000 µmol(photon).m-2.s-1

Custom Defined Protocols:

Variable timing, special language and scripts

A/D Bit Resolution:

16 bit

Detector time response:

1 µs in FL 3500-F

4 µs in FL 3500-S

Communication Port:

USB 2.0

Weight:

5 kg

Dimensions:

29 x 20 x 11 cm (Control Box)

Diameter 16 cm, height 6 cm (SuperHead Measuring Unit)

Power Input:

20 W

Electrical:

90 - 240 V

Warranty:

1 year parts and labor