Closed GFPCam FC 800-C/1010-GFP is a GFP version of the Closed FluorCam and represents a highly innovative, robust and user-friendly world-wide used system for combined multispectral and kinetic fluorescence imaging. The basic system of the Closed FluorCam (CCD camera, four fixed LED panels) is supplemented by a fully motorized and software-controlled filter wheel and appropriate filter sets for detection and imaging of green fluorescent protein (GFP), red-shifted GFP (EGFP), or other variants of fluorescent proteins. The blue emitting and red fluorescent protein can be detected and imaged in absence of chlorophyll.
The GFPCam consists of a CCD camera, four to five fixed LED panels (4 + 1 additional, which is not included in the standard setup) and of a filter wheel equipped with up to 7 different emission filters. The LED panels provide uniform irradiance over sample area – suitable for imaging of small plants (such as Arabidopsis thaliana), detached leaves, mosses, lichens, plated algal colonies or algal suspensions, etc. The system is very compact and allows easy dark adaptation of an investigated sample
The Closed GFPCam generates images of fluorescence signal at any moment of the experiment and presents them using a false color scale. Full kinetic analysis is available. In all applications, the camera allows imaging of fluorescence transients that are induced by actinic light or by saturating flashes. The timing and amplitude of actinic irradiance are determined by user-defined protocols. The device configuration also provides user-friendly and quick switching between the GFP and chlorophyll fluorescence detection.
The GFPCam includes a high-performance PC and comprehensive software package comprising full system control, data acquisition and image processing. For an experienced professional, the software offers a sophisticated programming language that can be used for designing novel timing and measuring sequences.